› 3q5e

X-ray diffraction
3.01Å resolution

crystal structure of human Atlastin-1 (residues 1-447) bound to GDP, crystal form 2

Released:
DOI: 10.2210/pdb3q5e/pdb
PDB entry 3q5e coloured by chain, front view.
PDB entry 3q5e coloured by chain, side view.
PDB entry 3q5e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A.
Byrnes LJ, Sondermann H
Proc Natl Acad Sci U S A 108 2216-21 (2011)
PMID: 21220294

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Atlastin-1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 447 amino acids
Theoretical weight: 51.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WXF7 (Residues: 1-447; Coverage: 80%)
Gene names: ATL1, GBP3, SPG3A
Sequence domains: Guanylate-binding protein, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 4 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 104.394Å b: 133.549Å c: 175.835Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

Dynamin, a membrane-remodelling GTPase.
Ferguson et al. (2012)
31 more

13 mentions without citation

Invited review: Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily.
Daumke et al. (2016)
12 more