› 3q5f

X-ray diffraction
2.96Å resolution

Crystal structure of the Salmonella transcriptional regulator SlyA in complex with DNA

Released:
DOI: 10.2210/pdb3q5f/pdb
PDB entry 3q5f coloured by chain, front view.
PDB entry 3q5f coloured by chain, side view.
PDB entry 3q5f coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structures of SlyA protein, a master virulence regulator of Salmonella, in free and DNA-bound states.
Dolan KT, Duguid EM, He C
J Biol Chem 286 22178-85 (2011)
PMID: 21550983

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116741 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator SlyA Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.76 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P40676 (Residues: 1-144; Coverage: 100%)
Gene names: STM1444, slyA
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*AP*TP*AP*AP*CP*TP*TP*AP*GP*CP*AP*AP*GP*CP*TP*AP*AP*TP*TP*AP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.06 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Not provided
DNA (5'-D(*TP*TP*AP*TP*AP*AP*TP*TP*AP*GP*CP*TP*TP*GP*CP*TP*AP*AP*GP*TP*TP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.06 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P41212
Unit cell:
a: 59.566Å b: 59.566Å c: 291.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.214 0.266
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Thermosensing to adjust bacterial virulence in a fluctuating environment.
Steinmann et al. (2013)
8 more

10 mentions without citation

Crystal structures of SlyA protein, a master virulence regulator of Salmonella, in free and DNA-bound states.
Dolan et al. (2011)
9 more