› 3q79

X-ray diffraction
2.51Å resolution

Cryptococcus neoformans protein farnesyltransferase in complex with farnesyl-DDPTASACNIQ product

Released:
DOI: 10.2210/pdb3q79/pdb
PDB entry 3q79 coloured by chain, front view.
PDB entry 3q79 coloured by chain, side view.
PDB entry 3q79 coloured by chain, top view.
Source organism: Cryptococcus neoformans var. grubii H99
Primary publication:
Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens.
Hast MA, Nichols CB, Armstrong SM, Kelly SM, Hellinga HW, Alspaugh JA, Beese LS
J Biol Chem 286 35149-62 (2011)
PMID: 21816822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241335 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Farnesyltransferase alpha subunit Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.91 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli
Structure domains: Protein prenylyltransferase
Farnesyltransferase beta subunit Chain: B
Molecule details ›
Chain: B
Length: 520 amino acids
Theoretical weight: 56.81 KDa
Source organism: Cryptococcus neoformans var. grubii H99
Expression system: Escherichia coli
Structure domains: Glycosyltransferase
isoprenylated product Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.13 KDa

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand FAR 1 x FAR
Ligand SO4 1 x SO4
Ligand 3CX 3 x 3CX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P43212
Unit cell:
a: 141.9Å b: 141.9Å c: 130.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.258
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Antifungal Drug Resistance: Molecular Mechanisms in Candida albicans and Beyond.
Lee et al. (2021)
12 more

1 mention without citation

Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens.
Hast et al. (2011)