› 3q7v

X-ray diffraction
2.1Å resolution

Beta-Lactam-Sensor Domain of BlaR1 (Apo) from Staphylococcus Aureus with Carboxylated Lys392

Released:
DOI: 10.2210/pdb3q7v/pdb
Model geometry
Fit model/data
PDB entry 3q7v coloured by chain, front view.
PDB entry 3q7v coloured by chain, side view.
PDB entry 3q7v coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus.
Borbulevych O, Kumarasiri M, Wilson B, Llarrull LI, Lee M, Hesek D, Shi Q, Peng J, Baker BM, Mobashery S
J Biol Chem 286 31466-72 (2011)
PMID: 21775440

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182143 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactam sensor/signal transducer BlaR1 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 29.59 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WU28 (Residues: 332-583; Coverage: 43%)
Gene names: GF599_11070, VRA0048, blaR1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily
Beta-lactam sensor/signal transducer BlaR1 Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 29.63 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WU28 (Residues: 332-583; Coverage: 43%)
Gene names: GF599_11070, VRA0048, blaR1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 46.955Å b: 107.43Å c: 56.496Å
α: 90° β: 108.75° γ: 90°
R-values:
R R work R free
0.176 0.173 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial cell-wall recycling.
Johnson et al. (2013)
4 more

5 mentions without citation

Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus.
Borbulevych et al. (2011)
4 more