› 3q9o

X-ray diffraction
1.79Å resolution

Full-length Cholix toxin from Vibrio cholerae in complex with NAD

Released:
DOI: 10.2210/pdb3q9o/pdb
PDB entry 3q9o coloured by chain, front view.
PDB entry 3q9o coloured by chain, side view.
PDB entry 3q9o coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The 1.8 Å cholix toxin crystal structure in complex with NAD+ and evidence for a new kinetic model.
Fieldhouse RJ, Jørgensen R, Lugo MR, Merrill AR
J Biol Chem 287 21176-88 (2012)
PMID: 22535961

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholix toxin Chain: A
Molecule details ›
Chain: A
Length: 642 amino acids
Theoretical weight: 71.87 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EK40 (Residues: 33-666; Coverage: 100%)
Gene names: chxA, toxA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 50.929Å b: 89.083Å c: 80.283Å
α: 90° β: 95.11° γ: 90°
R-values:
R R work R free
0.165 0.163 0.204
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural biology of the writers, readers, and erasers in mono- and poly(ADP-ribose) mediated signaling.
Karlberg et al. (2013)
2 more

4 mentions without citation

Novel bacterial ADP-ribosylating toxins: structure and function.
Simon et al. (2014)
3 more