› 3qbx

X-ray diffraction
2.1Å resolution

Crystal structure of pseudomonas aeruginosa 1,6-anhydro-n-actetylmuramic acid kinase (ANMK) bound to 1,6-anhydro-n-actetylmuramic acid

Released:
DOI: 10.2210/pdb3qbx/pdb
PDB entry 3qbx coloured by chain, front view.
PDB entry 3qbx coloured by chain, side view.
PDB entry 3qbx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Molecular basis of 1,6-anhydro bond cleavage and phosphoryl transfer by Pseudomonas aeruginosa 1,6-anhydro-N-acetylmuramic acid kinase.
Bacik JP, Whitworth GE, Stubbs KA, Yadav AK, Martin DR, Bailey-Elkin BA, Vocadlo DJ, Mark BL
J Biol Chem 286 12283-91 (2011)
PMID: 21288904

Function and Biology Details

Reaction catalysed: 
ATP + 1,6-anhydro-N-acetyl-beta-muramate + H(2)O = ADP + N-acetylmuramate 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydro-N-acetylmuramic acid kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 40.13 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I5Q5 (Residues: 1-363; Coverage: 100%)
Gene names: PA0666, anmK
Sequence domains: Anhydro-N-acetylmuramic acid kinase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand AH0 2 x AH0
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P63
Unit cell:
a: 91.48Å b: 91.48Å c: 173.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.215
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bacterial cell-wall recycling.
Johnson et al. (2013)
5 more

1 mention without citation

Molecular basis of 1,6-anhydro bond cleavage and phosphoryl transfer by Pseudomonas aeruginosa 1,6-anhydro-N-acetylmuramic acid kinase.
Bacik et al. (2011)