› 3qc9

X-ray diffraction
2.7Å resolution

Crystal structure of cross-linked bovine GRK1 T8C/N480C double mutant complexed with ADP and Mg

Released:
DOI: 10.2210/pdb3qc9/pdb
PDB entry 3qc9 coloured by chain, front view.
PDB entry 3qc9 coloured by chain, side view.
PDB entry 3qc9 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Activation of G protein-coupled receptor kinase 1 involves interactions between its N-terminal region and its kinase domain.
Huang CC, Orban T, Jastrzebska B, Palczewski K, Tesmer JJ
Biochemistry 50 1940-9 (2011)
PMID: 21265573

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin kinase GRK1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 543 amino acids
Theoretical weight: 61.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28327 (Residues: 1-535; Coverage: 95%)
Gene names: GRK1, RHOK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 8 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 58.696Å b: 89.965Å c: 122.638Å
α: 88.07° β: 90.26° γ: 68.78°
R-values:
R R work R free
0.194 0.192 0.229
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

G protein-coupled receptor kinases: more than just kinases and not only for GPCRs.
Gurevich et al. (2012)
8 more

1 mention without citation

Structural and functional analysis of g protein-coupled receptor kinase inhibition by paroxetine and a rationally designed analog.
Homan et al. (2014)