3qd6

X-ray diffraction
3.5Å resolution

Crystal structure of the CD40 and CD154 (CD40L) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-150623 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CD40 ligand, soluble form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 149 amino acids
Theoretical weight: 16.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P29965 (Residues: 116-261; Coverage: 56%)
Gene names: CD40L, CD40LG, TNFSF5, TRAP
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Tumor necrosis factor receptor superfamily member 5 Chains: R, S, T, U
Molecule details ›
Chains: R, S, T, U
Length: 177 amino acids
Theoretical weight: 19.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P25942 (Residues: 21-190; Coverage: 66%)
Gene names: CD40, TNFRSF5
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 133.212Å b: 133.212Å c: 211.151Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.245 0.298
Expression system: Trichoplusia ni