› 3qd9

X-ray diffraction
3.3Å resolution

C72S/C353S mutant of Trypanosoma brucei QSOX containing an interdomain disulfide

Released:
DOI: 10.2210/pdb3qd9/pdb
PDB entry 3qd9 coloured by chain, front view.
PDB entry 3qd9 coloured by chain, side view.
PDB entry 3qd9 coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
The dynamic disulphide relay of quiescin sulphydryl oxidase.
Alon A, Grossman I, Gat Y, Kodali VK, DiMaio F, Mehlman T, Haran G, Baker D, Thorpe C, Fass D
Nature 488 414-8 (2012)
PMID: 22801504

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfhydryl oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 52.21 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q585M6 (Residues: 20-485; Coverage: 92%)
Gene names: Tb06.28P18.860, Tb927.6.1850
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 81.04Å b: 120.31Å c: 106.22Å
α: 90° β: 105.44° γ: 90°
R-values:
R R work R free
0.196 0.19 0.249
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Proinsulin misfolding and endoplasmic reticulum stress during the development and progression of diabetes.
Sun et al. (2015)
12 more

5 mentions without citation

The dynamic disulphide relay of quiescin sulphydryl oxidase.
Alon et al. (2012)
4 more