› 3qff

X-ray diffraction
1.96Å resolution

Crystal Structure of ADP complex of purK: N5-carboxyaminoimidazole ribonucleotide synthetase

Released:
DOI: 10.2210/pdb3qff/pdb
PDB entry 3qff coloured by chain, front view.
PDB entry 3qff coloured by chain, side view.
PDB entry 3qff coloured by chain, top view.
Source organism: Bacillus anthracis str. A0248
Primary publication:
Elucidation of the bicarbonate binding site and insights into the carboxylation mechanism of (N(5))-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.
Tuntland ML, Santarsiero BD, Johnson ME, Fung LW
Acta Crystallogr D Biol Crystallogr 70 3057-65 (2014)
PMID: 25372694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.21 KDa
Source organism: Bacillus anthracis str. A0248
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 57.421Å b: 82.089Å c: 166.768Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Catalytic and structural properties of ATP-dependent caprolactamase from Pseudomonas jessenii.
Marjanovic et al. (2021)