› 3qfo

X-ray diffraction
2.2Å resolution

Crystal structure of Streptococcal asymmetric Ap4A hydrolase and phosphodiesterase Spr1479/SapH im complex with AMP

Released:
DOI: 10.2210/pdb3qfo/pdb
PDB entry 3qfo coloured by chain, front view.
PDB entry 3qfo coloured by chain, side view.
PDB entry 3qfo coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Entry authors: Jiang YL, Zhang JW, Yu WL, Cheng W, Zhang CC, Zhou CZ, Chen Y

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcineurin-like phosphoesterase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 31.42 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DNX4 (Residues: 1-262; Coverage: 93%)
Gene name: spr1479
Sequence domains: Calcineurin-like phosphoesterase superfamily domain
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand AMP 2 x AMP
Ligand FE 2 x FE
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 57.654Å b: 74.94Å c: 149.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.245
Expression system: Escherichia coli

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