› 3qfq

X-ray diffraction
2.9Å resolution

Asymmetric Assembly of Merkel Cell Polyomavirus Large T-antigen Origin Binding Domains at the Viral Origin

Released:
DOI: 10.2210/pdb3qfq/pdb
PDB entry 3qfq coloured by chain, front view.
PDB entry 3qfq coloured by chain, side view.
PDB entry 3qfq coloured by chain, top view.
Source organism: Merkel cell polyomavirus
Primary publication:
Asymmetric assembly of Merkel cell polyomavirus large T-antigen origin binding domains at the viral origin.
Harrison CJ, Meinke G, Kwun HJ, Rogalin H, Phelan PJ, Bullock PA, Chang Y, Moore PS, Bohm A
J Mol Biol 409 529-42 (2011)
PMID: 21501625

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116767 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 135 amino acids
Theoretical weight: 15.64 KDa
Source organism: Merkel cell polyomavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E2IPT4 (Residues: 230-355; Coverage: 17%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,
DNA (26-MER) Chain: W
Molecule details ›
Chain: W
Length: 26 nucleotides
Theoretical weight: 7.98 KDa
Source organism: Merkel cell polyomavirus
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: Merkel cell polyomavirus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 80.4Å b: 171.34Å c: 51.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Merkel cell carcinoma.
Becker et al. (2017)
10 more

3 mentions without citation

Asymmetric assembly of Merkel cell polyomavirus large T-antigen origin binding domains at the viral origin.
Harrison et al. (2011)
2 more