3qg5

X-ray diffraction
3.4Å resolution

The Mre11:Rad50 complex forms an ATP dependent molecular clamp in DNA double-strand break repair

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-194848 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA double-strand break repair Rad50 ATPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.26 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1X1 (Residues: 1-190, 686-852; Coverage: 42%)
Gene names: TM_1636, rad50
Sequence domains:
Structure domains:
DNA double-strand break repair protein Mre11 Chains: C, D
Molecule details ›
Chains: C, D
Length: 379 amino acids
Theoretical weight: 44.32 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1X0 (Residues: 7-385; Coverage: 98%)
Gene names: TM_1635, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 105.19Å b: 187Å c: 300.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.258 0.295
Expression system: Escherichia coli