› 3qhq

X-ray diffraction
2Å resolution

Structure of CRISPR-associated protein Csn2

Released:
DOI: 10.2210/pdb3qhq/pdb
PDB entry 3qhq coloured by chain, front view.
PDB entry 3qhq coloured by chain, side view.
PDB entry 3qhq coloured by chain, top view.
Source organism: Streptococcus agalactiae ATCC 13813
Entry authors: Ellinger P, Arslan Z, Wurm R, Tschapek B, Pfeffer K, Wagner R, Schmitt L, Pul U, Smits SH

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein Csn2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.73 KDa
Source organism: Streptococcus agalactiae ATCC 13813
Expression system: Escherichia coli
UniProt:
  • Canonical: E7S4M0 (Residues: 1-221; Coverage: 100%)
Gene names: HMPREF9171_1216, csn2
Sequence domains: CRISPR-associated protein (Cas_Csn2)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 75.3Å b: 83.3Å c: 110.4Å
α: 90° β: 109.4° γ: 90°
R-values:
R R work R free
0.205 0.204 0.228
Expression system: Escherichia coli

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