› 3qid

X-ray diffraction
2.5Å resolution

Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase

Released:
DOI: 10.2210/pdb3qid/pdb
PDB entry 3qid coloured by chain, front view.
PDB entry 3qid coloured by chain, side view.
PDB entry 3qid coloured by chain, top view.
Source organism: Murine norovirus 1
Primary publication:
Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase.
Lee JH, Alam I, Han KR, Cho S, Shin S, Kang S, Yang JM, Kim KH
J Gen Virol 92 1607-1616 (2011)
PMID: 21471315

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 517 amino acids
Theoretical weight: 58.52 KDa
Source organism: Murine norovirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80J95 (Residues: 1181-1686; Coverage: 30%)
Gene name: ORF1
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 28 x SO4
Ligand MN3 3 x MN3
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 120.557Å b: 196.262Å c: 109.339Å
α: 90° β: 114.23° γ: 90°
R-values:
R R work R free
0.202 0.199 0.245
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Antiviral targets of human noroviruses.
Prasad et al. (2016)
7 more

3 mentions without citation

Structure and Function of Caliciviral RNA Polymerases.
Lee et al. (2017)
2 more