3qjj

X-ray diffraction
2.8Å resolution

One RAMP protein binding different RNA substrates

Released:
Source organism: Pyrococcus horikoshii
Entry authors: Wang R, Zheng H, Preamplume G, Li H

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-129669 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Putative CRISPR-associated endoribonuclease-like protein Cas6nc Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 28.07 KDa
Source organism: Pyrococcus horikoshii
Expression system: Not provided
UniProt:
  • Canonical: O58088 (Residues: 1-239; Coverage: 100%)
Gene names: PH0350, cas6nc
Sequence domains:
Structure domains:
RNA (5'-R(*GP*UP*UP*GP*AP*AP*AP*UP*CP*AP*GP*A)-3') Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 12 nucleotides
Theoretical weight: 3.86 KDa
Source organism: Pyrococcus horikoshii
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 75.269Å b: 75.269Å c: 257.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.211 0.262
Expression system: Not provided