› 3qk1

X-ray diffraction
2.08Å resolution

Crystal Structure of Enterokinase-like Trypsin Variant

Released:
DOI: 10.2210/pdb3qk1/pdb
PDB entry 3qk1 coloured by chain, front view.
PDB entry 3qk1 coloured by chain, side view.
PDB entry 3qk1 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Towards a restriction proteinase: construction of a self-activating enzyme.
Schöpfel M, Tziridis A, Arnold U, Stubbs MT
Chembiochem 12 1523-7 (2011)
PMID: 21387509

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.31 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

6 bound ligands:
Ligand CA 1 x CA
Ligand SO4 3 x SO4
Ligand EDO 4 x EDO
Ligand IMD 1 x IMD
Ligand GOL 1 x GOL
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 60.71Å b: 63.76Å c: 69.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.182 0.237
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.
Currin et al. (2015)
1 other article

1 mention without citation

Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes.
Yin et al. (2014)