› 3qk9

X-ray diffraction
3.1Å resolution

Yeast Tim44 C-terminal domain complexed with Cymal-3

Released:
DOI: 10.2210/pdb3qk9/pdb
PDB entry 3qk9 coloured by chain, front view.
PDB entry 3qk9 coloured by chain, side view.
PDB entry 3qk9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Membrane binding mechanism of yeast mitochondrial peripheral membrane protein TIM44.
Cui W, Josyula R, Li J, Fu Z, Sha B
Protein Pept Lett 18 718-25 (2011)
PMID: 21342097

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial import inner membrane translocase subunit TIM44 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01852 (Residues: 210-431; Coverage: 52%)
Gene names: ISP45, MIM44, MPI1, TIM44, YIL022W
Sequence domains: Tim44-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-BM-B
Spacegroup: P6522
Unit cell:
a: 100.595Å b: 100.595Å c: 209.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.312 0.235 0.317
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Architecture of the large subunit of the mammalian mitochondrial ribosome.
Greber et al. (2014)
1 more

1 mention without citation

Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44.
Miyata et al. (2017)