› 3ql1

X-ray diffraction
1.29Å resolution

Crystal Structure of Ribonuclease A Variant A4C/D83E/V118C

Released:
DOI: 10.2210/pdb3ql1/pdb
PDB entry 3ql1 coloured by chain, front view.
PDB entry 3ql1 coloured by chain, side view.
PDB entry 3ql1 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Significant stabilization of ribonuclease A by additive effects.
Arnold U, Schöpfel M
FEBS J 279 2508-19 (2012)
PMID: 22594773

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.76 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand EDO 1 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 54.248Å b: 53.781Å c: 42.869Å
α: 90° β: 117.95° γ: 90°
R-values:
R R work R free
0.156 0.154 0.19
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Stability and folding of amphibian ribonuclease A superfamily members in comparison with mammalian homologues.
Arnold U. (2014)
3 other articles