› 3qlb

X-ray diffraction
3.26Å resolution

Enantiopyochelin outer membrane TonB-dependent transporter from Pseudomonas fluorescens bound to the ferri-enantiopyochelin

Released:
DOI: 10.2210/pdb3qlb/pdb
PDB entry 3qlb coloured by chain, front view.
PDB entry 3qlb coloured by chain, side view.
PDB entry 3qlb coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Pyochelin enantiomers and their outer-membrane siderophore transporters in fluorescent pseudomonads: structural bases for unique enantiospecific recognition.
Brillet K, Reimmann C, Mislin GL, Noël S, Rognan D, Schalk IJ, Cobessi D
J Am Chem Soc 133 16503-9 (2011)
PMID: 21902256

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FetA Chains: A, B
Molecule details ›
Chains: A, B
Length: 748 amino acids
Theoretical weight: 81.84 KDa
Source organism: Pseudomonas fluorescens
Expression system: Pseudomonas fluorescens
UniProt:
  • Canonical: C5I2D9 (Residues: 1-437, 438-743; Coverage: 100%)
Gene name: fetA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand CIT 2 x CIT
Ligand EFE 2 x EFE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2221
Unit cell:
a: 155.07Å b: 170.83Å c: 232.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.259
Expression system: Pseudomonas fluorescens

Quick links

Citations

3 review citations

Siderophore-dependent iron uptake systems as gates for antibiotic Trojan horse strategies against Pseudomonas aeruginosa.
Mislin et al. (2014)
2 more

1 mention without citation

In silico determination and validation of baumannii acinetobactin utilization a structure and ligand binding site.
Sefid et al. (2013)