› 3qmv

X-ray diffraction
2.12Å resolution

RedJ-Thioesterase from the Prodiginine biosynthetic pathway in Streptomyces coelicolor

Released:
DOI: 10.2210/pdb3qmv/pdb
PDB entry 3qmv coloured by chain, front view.
PDB entry 3qmv coloured by chain, side view.
PDB entry 3qmv coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure and function of the RedJ protein, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor.
Whicher JR, Florova G, Sydor PK, Singh R, Alhamadsheh M, Challis GL, Reynolds KA, Smith JL
J Biol Chem 286 22558-69 (2011)
PMID: 21543318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase thioesterase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 31.3 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: O54157 (Residues: 6-261; Coverage: 91%)
Gene names: SC3F7.14, SCO5894, redJ
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 74.813Å b: 79.399Å c: 87.438Å
α: 90° β: 90.12° γ: 90°
R-values:
R R work R free
0.203 0.201 0.249
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Exploitation of the Streptomyces coelicolor A3(2) genome sequence for discovery of new natural products and biosynthetic pathways.
Challis GL. (2014)
6 more

5 mentions without citation

Structure and function of the RedJ protein, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor.
Whicher et al. (2011)
4 more