› 3qof

X-ray diffraction
2.8Å resolution

Crystal structure of the cytosolic domain of human atlastin-1 in complex with GDP, orthorhombic form

Released:
DOI: 10.2210/pdb3qof/pdb
PDB entry 3qof coloured by chain, front view.
PDB entry 3qof coloured by chain, side view.
PDB entry 3qof coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the atlastin GTPase provide insight into homotypic fusion of endoplasmic reticulum membranes.
Bian X, Klemm RW, Liu TY, Zhang M, Sun S, Sui X, Liu X, Rapoport TA, Hu J
Proc Natl Acad Sci U S A 108 3976-81 (2011)
PMID: 21368113

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Atlastin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 459 amino acids
Theoretical weight: 52.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WXF7 (Residues: 18-447; Coverage: 77%)
Gene names: ATL1, GBP3, SPG3A
Sequence domains: Guanylate-binding protein, N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 4 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 104.742Å b: 133.445Å c: 176.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.225 0.267
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Dynamin, a membrane-remodelling GTPase.
Ferguson et al. (2012)
40 more

6 mentions without citation

Weaving the web of ER tubules.
Hu et al. (2011)
5 more