› 3qp5

X-ray diffraction
3.25Å resolution

Crystal structure of CviR bound to antagonist chlorolactone (CL)

Released:
DOI: 10.2210/pdb3qp5/pdb
PDB entry 3qp5 coloured by chain, front view.
PDB entry 3qp5 coloured by chain, side view.
PDB entry 3qp5 coloured by chain, top view.
Source organism: Chromobacterium violaceum
Primary publication:
A strategy for antagonizing quorum sensing.
Chen G, Swem LR, Swem DL, Stauff DL, O'Loughlin CT, Jeffrey PD, Bassler BL, Hughson FM
Mol Cell 42 199-209 (2011)
PMID: 21504831

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CviR transcriptional regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 29.75 KDa
Source organism: Chromobacterium violaceum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3W065 (Residues: 1-265; Coverage: 100%)
Gene name: cviR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HLC 4 x HLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 169.318Å b: 169.318Å c: 99.844Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.263 0.304
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

Bacterial quorum sensing: its role in virulence and possibilities for its control.
Rutherford et al. (2012)
32 more

28 mentions without citation

Quorum sensing signal-response systems in Gram-negative bacteria.
Papenfort et al. (2016)
27 more