› 3qpd

X-ray diffraction
1.57Å resolution

Structure of Aspergillus oryzae cutinase expressed in Pichia pastoris, crystallized in the presence of Paraoxon

Released:
DOI: 10.2210/pdb3qpd/pdb
PDB entry 3qpd coloured by chain, front view.
PDB entry 3qpd coloured by chain, side view.
PDB entry 3qpd coloured by chain, top view.
Source organism: Aspergillus oryzae
Entry authors: Lu A, Gosser Y, Montclare JK, Liu Z, Kong X

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase 1 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 19.62 KDa
Source organism: Aspergillus oryzae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P52956 (Residues: 26-212; Coverage: 95%)
Gene names: AO090005000029, CutL1, cutL
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P3221
Unit cell:
a: 45.078Å b: 45.078Å c: 157.711Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.184
Expression system: Komagataella pastoris

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