› 3qqm

X-ray diffraction
2.3Å resolution

Crystal structure of a Putative amino-acid aminotransferase (NP_104211.1) from Mesorhizobium loti at 2.30 A resolution

Released:
DOI: 10.2210/pdb3qqm/pdb
PDB entry 3qqm coloured by chain, front view.
PDB entry 3qqm coloured by chain, side view.
PDB entry 3qqm coloured by chain, top view.
Source organism: Mesorhizobium loti
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable branched-chain-amino-acid aminotransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 221 amino acids
Theoretical weight: 24.75 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98H69 (Residues: 1-220; Coverage: 100%)
Gene name: mlr3007
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand IOD 26 x IOD
Ligand CL 14 x CL
Ligand EDO 50 x EDO
Ligand PEG 1 x PEG
2 modified residues:
Ligand MSE 24 x MSE
Ligand LLP 8 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 116.178Å b: 215.574Å c: 81.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.241
Expression system: Escherichia coli

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