› 3qqr

X-ray diffraction
2.16Å resolution

Crystal structure of Parasponia hemoglobin; Differential Heme Coordination is Linked to Quaternary Structure

Released:
DOI: 10.2210/pdb3qqr/pdb
PDB entry 3qqr coloured by chain, front view.
PDB entry 3qqr coloured by chain, side view.
PDB entry 3qqr coloured by chain, top view.
Source organism: Parasponia andersonii
Primary publication:
Crystal structures of Parasponia and Trema hemoglobins: differential heme coordination is linked to quaternary structure.
Kakar S, Sturms R, Tiffany A, Nix JC, DiSpirito AA, Hargrove MS
Biochemistry 50 4273-80 (2011)
PMID: 21491905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaerobic nitrite reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.2 KDa
Source organism: Parasponia andersonii
Expression system: Escherichia coli
UniProt:
  • Canonical: P68168 (Residues: 1-162; Coverage: 100%)
Gene name: HB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand DIO 5 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 55.04Å b: 72.04Å c: 88.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.224 0.286
Expression system: Escherichia coli

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Citations

2 review citations

Plant hemoglobins: important players at the crossroads between oxygen and nitric oxide.
Gupta et al. (2011)
1 more