› 3qqw

X-ray diffraction
2.44Å resolution

Crystal structure of a putative lyase (Reut_B4148) from Ralstonia eutropha JMP134 at 2.44 A resolution

Released:
DOI: 10.2210/pdb3qqw/pdb
PDB entry 3qqw coloured by chain, front view.
PDB entry 3qqw coloured by chain, side view.
PDB entry 3qqw coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HpcH/HpaI aldolase/citrate lyase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 332 amino acids
Theoretical weight: 36.54 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46TN2 (Residues: 1-331; Coverage: 100%)
Gene name: Reut_B4148
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

1 bound ligand:
Ligand CL 9 x CL
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 226.401Å b: 73.826Å c: 149.252Å
α: 90° β: 125.31° γ: 90°
R-values:
R R work R free
0.203 0.202 0.231
Expression system: Escherichia coli

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