› 3qqy

X-ray diffraction
2.4Å resolution

Crystal structure of a novel LAGLIDADG homing endonuclease, I-OnuI (from Ophiostoma novo-ulmi subsp. americana)

Released:
DOI: 10.2210/pdb3qqy/pdb
PDB entry 3qqy coloured by chain, front view.
PDB entry 3qqy coloured by chain, side view.
PDB entry 3qqy coloured by chain, top view.
Source organism: Ophiostoma novo-ulmi subsp. americana
Primary publication:
Tapping natural reservoirs of homing endonucleases for targeted gene modification.
Takeuchi R, Lambert AR, Mak AN, Jacoby K, Dickson RJ, Gloor GB, Scharenberg AM, Edgell DR, Stoddard BL
Proc Natl Acad Sci U S A 108 13077-82 (2011)
PMID: 21784983

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116780 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 35.17 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4VWW5 (Residues: 413-715; Coverage: 42%)
Gene names: HEG fusion, rps3
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 7.76 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 8.21 KDa
Source organism: Ophiostoma novo-ulmi subsp. americana
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 37.948Å b: 73.933Å c: 166.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

Genome engineering with targetable nucleases.
Carroll D. (2014)
16 more

12 mentions without citation

Tapping natural reservoirs of homing endonucleases for targeted gene modification.
Takeuchi et al. (2011)
11 more