› 3qr0

X-ray diffraction
2Å resolution

Crystal Structure of S. officinalis PLC21

Released:
DOI: 10.2210/pdb3qr0/pdb
PDB entry 3qr0 coloured by chain, front view.
PDB entry 3qr0 coloured by chain, side view.
PDB entry 3qr0 coloured by chain, top view.
Source organism: Sepia officinalis
Primary publication:
An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation.
Lyon AM, Tesmer VM, Dhamsania VD, Thal DM, Gutierrez J, Chowdhury S, Suddala KC, Northup JK, Tesmer JJ
Nat Struct Mol Biol 18 999-1005 (2011)
PMID: 21822282

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 816 amino acids
Theoretical weight: 92.87 KDa
Source organism: Sepia officinalis
UniProt:
  • Canonical: F2YQ19 (Residues: 1-816; Coverage: 72%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 60.815Å b: 89.396Å c: 163.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.208

Quick links

Citations

13 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
12 more

5 mentions without citation

Structural insights into phospholipase C-β function.
Lyon et al. (2013)
4 more