› 3qr1

X-ray diffraction
3.2Å resolution

Crystal Structure of L. pealei PLC21

Released:
DOI: 10.2210/pdb3qr1/pdb
Model geometry
Fit model/data
PDB entry 3qr1 coloured by chain, front view.
PDB entry 3qr1 coloured by chain, side view.
PDB entry 3qr1 coloured by chain, top view.
Source organism: Doryteuthis pealeii
Primary publication:
An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation.
Lyon AM, Tesmer VM, Dhamsania VD, Thal DM, Gutierrez J, Chowdhury S, Suddala KC, Northup JK, Tesmer JJ
OpenAccess logo Nat Struct Mol Biol 18 999-1005 (2011)
PMID: 21822282

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase Chains: A, D
Molecule details ›
Chains: A, D
Length: 813 amino acids
Theoretical weight: 92.41 KDa
Source organism: Doryteuthis pealeii
UniProt:
  • Canonical: Q9NBA8 (Residues: 1-813; Coverage: 72%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 82.367Å b: 148.869Å c: 151.572Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.258

Quick links

Citations

13 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
12 more

4 mentions without citation

Structural insights into phospholipase C-β function.
Lyon et al. (2013)
3 more