› 3qse

X-ray diffraction
1.75Å resolution

Crystal structure for the complex of substrate-reduced msox with sarcosine

Released:
DOI: 10.2210/pdb3qse/pdb
PDB entry 3qse coloured by chain, front view.
PDB entry 3qse coloured by chain, side view.
PDB entry 3qse coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Kommoju PR, Chen ZW, Bruckner RC, Mathews FS, Jorns MS
Biochemistry 50 5521-34 (2011)
PMID: 21568312

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli DH1
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand CL 2 x CL
Ligand SAR 2 x SAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 71.172Å b: 69.724Å c: 72.945Å
α: 90° β: 92.32° γ: 90°
R-values:
R R work R free
0.184 0.184 0.217
Expression system: Escherichia coli DH1

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Citations

1 review citation

Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.
Bauer et al. (2022)
24 other articles