› 3qsm

X-ray diffraction
1.9Å resolution

Crystal structure for the MSOX.chloride binary complex

Released:
DOI: 10.2210/pdb3qsm/pdb
PDB entry 3qsm coloured by chain, front view.
PDB entry 3qsm coloured by chain, side view.
PDB entry 3qsm coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Kommoju PR, Chen ZW, Bruckner RC, Mathews FS, Jorns MS
Biochemistry 50 5521-34 (2011)
PMID: 21568312

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli DH1
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 70.9Å b: 69.283Å c: 72.909Å
α: 90° β: 92.27° γ: 90°
R-values:
R R work R free
0.184 0.184 0.212
Expression system: Escherichia coli DH1

Quick links

Citations

1 review citation

Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.
Bauer et al. (2022)
23 other articles

1 mention without citation

X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction.
Shimasaki et al. (2017)