› 3qsu

X-ray diffraction
2.2Å resolution

Structure of Staphylococcus aureus Hfq in complex with A7 RNA

Released:
DOI: 10.2210/pdb3qsu/pdb
PDB entry 3qsu coloured by chain, front view.
PDB entry 3qsu coloured by chain, side view.
PDB entry 3qsu coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus ECT-R 2
Primary publication:
Structural mechanism of Staphylococcus aureus Hfq binding to an RNA A-tract.
Horstmann N, Orans J, Valentin-Hansen P, Shelburne SA, Brennan RG
Nucleic Acids Res 40 11023-35 (2012)
PMID: 22965117

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero heptamer (preferred)
homo hexamer
PDBe Complex ID:
PDB-CPX-163946 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA chaperone Hfq Chains: A, B, C, D, E, F, G, H, I, J, K, M, N, S
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, M, N, S
Length: 77 amino acids
Theoretical weight: 8.79 KDa
Source organism: Staphylococcus aureus subsp. aureus ECT-R 2
Expression system: Escherichia coli
Structure domains: SH3 type barrels.
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*A)-3') Chains: P, R
Molecule details ›
Chains: P, R
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Staphylococcus aureus subsp. aureus ECT-R 2
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 29 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3
Unit cell:
a: 156.397Å b: 156.397Å c: 34.646Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Hfq: the flexible RNA matchmaker.
Updegrove et al. (2016)
10 more

6 mentions without citation

Models of Hfq interactions with small non-coding RNA in Gram-negative and Gram-positive bacteria.
Watkins et al. (2023)
5 more