› 3qtc

X-ray diffraction
1.75Å resolution

Crystal structure of the catalytic domain of MmOmeRS, an O-methyl tyrosyl-tRNA synthetase evolved from Methanosarcina mazei PylRS, complexed with O-methyl tyrosine and AMP-PNP

Released:
DOI: 10.2210/pdb3qtc/pdb
PDB entry 3qtc coloured by chain, front view.
PDB entry 3qtc coloured by chain, side view.
PDB entry 3qtc coloured by chain, top view.
Source organism: Methanosarcina mazei
Primary publication:
Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids.
Takimoto JK, Dellas N, Noel JP, Wang L
ACS Chem Biol 6 733-43 (2011)
PMID: 21545173

Function and Biology Details

Reaction catalysed: 
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolysine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.5 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PWY1 (Residues: 185-454; Coverage: 60%)
Gene names: MM_1445, pylS
Sequence domains: tRNA synthetases class II core domain (F)
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand 0A1 1 x 0A1
Ligand EDO 5 x EDO
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P64
Unit cell:
a: 104.809Å b: 104.809Å c: 71.674Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.
Wan et al. (2014)
9 more

13 mentions without citation

Genetic Code Expansion in Animals.
Brown et al. (2018)
12 more