› 3qv2

X-ray diffraction
2.15Å resolution

Structure Analysis of Entamoeba histolytica methyltransferase EhMeth

Released:
DOI: 10.2210/pdb3qv2/pdb
PDB entry 3qv2 coloured by chain, front view.
PDB entry 3qv2 coloured by chain, side view.
PDB entry 3qv2 coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry authors: Schulz EC, Roth HM, Ankri S, Ficner R

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-cytosine DNA methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 37.87 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6Q386 (Residues: 1-322; Coverage: 100%)
Gene names: CL6EHI_069140, meth
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 46.97Å b: 46.97Å c: 303.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.267
Expression system: Escherichia coli BL21(DE3)

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