› 3qwr

X-ray diffraction
3.25Å resolution

Crystal structure of IL-23 in complex with an adnectin

Released:
DOI: 10.2210/pdb3qwr/pdb
PDB entry 3qwr coloured by chain, front view.
PDB entry 3qwr coloured by chain, side view.
PDB entry 3qwr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of adnectin/protein complexes reveal an expanded binding footprint.
Ramamurthy V, Krystek SR, Bush A, Wei A, Emanuel SL, Das Gupta R, Janjua A, Cheng L, Murdock M, Abramczyk B, Cohen D, Lin Z, Morin P, Davis JH, Dabritz M, McLaughlin DC, Russo KA, Chao G, Wright MC, Jenny VA, Engle LJ, Furfine E, Sheriff S
Structure 20 259-69 (2012)
PMID: 22325775

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151474 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-23 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NPF7 (Residues: 20-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha
Structure domains: Growth Hormone; Chain: A;
ADNECTIN Chain: D
Molecule details ›
Chain: D
Length: 109 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I212121
Unit cell:
a: 77.7Å b: 91.7Å c: 225.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.264
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

11 review citations

Non-immunoglobulin scaffolds: a focus on their targets.
Škrlec et al. (2015)
10 more

7 mentions without citation

Small-Format Drug Conjugates: A Viable Alternative to ADCs for Solid Tumours?
Deonarain et al. (2018)
6 more