› 3qxf

X-ray diffraction
1.85Å resolution

Structure of the bacterial cellulose synthase subunit Z

Released:
DOI: 10.2210/pdb3qxf/pdb
PDB entry 3qxf coloured by chain, front view.
PDB entry 3qxf coloured by chain, side view.
PDB entry 3qxf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Apo- and cellopentaose-bound structures of the bacterial cellulose synthase subunit BcsZ.
Mazur O, Zimmer J
J Biol Chem 286 17601-6 (2011)
PMID: 21454578

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 355 amino acids
Theoretical weight: 41.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37651 (Residues: 22-368; Coverage: 100%)
Gene names: JW3499, b3531, bcsC, bcsZ, yhjM
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 54.817Å b: 87.933Å c: 91.754Å
α: 69.96° β: 74.36° γ: 78.22°
R-values:
R R work R free
0.138 0.136 0.178
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions.
Römling et al. (2015)
3 more

6 mentions without citation

Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
Whitney et al. (2013)
5 more