› 3qxq

X-ray diffraction
2.2Å resolution

Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose

Released:
DOI: 10.2210/pdb3qxq/pdb
PDB entry 3qxq coloured by chain, front view.
PDB entry 3qxq coloured by chain, side view.
PDB entry 3qxq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Apo- and cellopentaose-bound structures of the bacterial cellulose synthase subunit BcsZ.
Mazur O, Zimmer J
J Biol Chem 286 17601-6 (2011)
PMID: 21454578

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 355 amino acids
Theoretical weight: 40.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37651 (Residues: 22-368; Coverage: 100%)
Gene names: JW3499, b3531, bcsC, bcsZ, yhjM
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 90.678Å b: 99.605Å c: 93.073Å
α: 90° β: 102.98° γ: 90°
R-values:
R R work R free
0.171 0.168 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A molecular description of cellulose biosynthesis.
McNamara et al. (2015)
2 more

7 mentions without citation

Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
Whitney et al. (2013)
6 more