› 3qy8

X-ray diffraction
2Å resolution

Crystal structures of YwqE from Bacillus subtilis and CpsB from Streptococcus pneumoniae, unique metal-dependent tyrosine phosphatases

Released:
DOI: 10.2210/pdb3qy8/pdb
PDB entry 3qy8 coloured by chain, front view.
PDB entry 3qy8 coloured by chain, side view.
PDB entry 3qy8 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Crystal structures of YwqE from Bacillus subtilis and CpsB from Streptococcus pneumoniae, unique metal-dependent tyrosine phosphatases.
Kim HS, Lee SJ, Yoon HJ, An DR, Kim DJ, Kim SJ, Suh SW
J Struct Biol 175 442-50 (2011)
PMID: 21605684

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase CpsB Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 29.25 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AHD4 (Residues: 1-243; Coverage: 100%)
Gene names: SP_0347, cpsB, wzh
Sequence domains: Capsular polysaccharide synthesis, CpsB/CapC
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand FE 2 x FE
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 42.16Å b: 61.634Å c: 111.662Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.233
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis.
Böhmer et al. (2013)
3 more