› 3qyf

X-ray diffraction
1.9Å resolution

Crystal structure of the CRISPR-associated protein SSO1393 from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb3qyf/pdb
PDB entry 3qyf coloured by chain, front view.
PDB entry 3qyf coloured by chain, side view.
PDB entry 3qyf coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Entry authors: Petit P, Xu X, Chang C, Savchenko A, Yakunin AF

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system ring nuclease SSO1393 Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 38.02 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97YD2 (Residues: 1-321; Coverage: 100%)
Gene name: SSO1393
Sequence domains: CRISPR-associated protein (Cas_APE2256)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 120.614Å b: 81.554Å c: 70.155Å
α: 90° β: 108.3° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression system: Escherichia coli BL21(DE3)

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