› 3r1x

X-ray diffraction
2.09Å resolution

Crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb3r1x/pdb
PDB entry 3r1x coloured by chain, front view.
PDB entry 3r1x coloured by chain, side view.
PDB entry 3r1x coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Primary publication:
Structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae.
Michalska K, Cuff ME, Tesar C, Feldmann B, Joachimiak A
Acta Crystallogr D Biol Crystallogr 67 678-89 (2011)
PMID: 21795809

Function and Biology Details

Reaction catalysed: 
ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-108093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxo-3-deoxygalactonate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 295 amino acids
Theoretical weight: 31.67 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A6TFZ6 (Residues: 1-292; Coverage: 100%)
Gene names: KPN_04097, dgoK
Sequence domains: 2-keto-3-deoxy-galactonokinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 4 x FMT
Ligand GOL 17 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 85.509Å b: 134.026Å c: 139.906Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.199
Expression system: Escherichia coli BL21

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Citations

3 citation in other articles

The Treponema denticola FhbB Protein Is a Dominant Early Antigen That Elicits FhbB Variant-Specific Antibodies That Block Factor H Binding and Cleavage by Dentilisin.
Miller et al. (2016)
2 more

2 mentions without citation

Target highlights in CASP9: Experimental target structures for the critical assessment of techniques for protein structure prediction.
Kryshtafovych et al. (2011)
1 more