› 3r3p

X-ray diffraction
2.2Å resolution

Homing Endonuclease I-Bth0305I Catalytic Domain

Released:
DOI: 10.2210/pdb3r3p/pdb
PDB entry 3r3p coloured by chain, front view.
PDB entry 3r3p coloured by chain, side view.
PDB entry 3r3p coloured by chain, top view.
Source organism: Bacillus phage 0305phi8-36
Primary publication:
Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.
Taylor GK, Heiter DF, Pietrokovski S, Stoddard BL
Nucleic Acids Res 39 9705-19 (2011)
PMID: 21890897

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-108279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF559 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 12.24 KDa
Source organism: Bacillus phage 0305phi8-36
Expression system: Escherichia coli
UniProt:
  • Canonical: A7KV39 (Residues: 167-266; Coverage: 38%)
Gene names: 0305phi8-36p029, ORF029
Sequence domains: Protein of unknown function (DUF559)
Structure domains: VSR Endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 52.292Å b: 52.292Å c: 133.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.261
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Homing endonucleases: DNA scissors on a mission.
Hafez et al. (2012)
3 more

6 mentions without citation

Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.
Taylor et al. (2011)
5 more