› 3r4t

X-ray diffraction
2.5Å resolution

Crystal structure of 4-aminobutyrate aminotransferase GabT from Mycobacterium marinum covalently bound to pyridoxal phosphate

Released:
DOI: 10.2210/pdb3r4t/pdb
Model geometry
Fit model/data
PDB entry 3r4t coloured by chain, front view.
PDB entry 3r4t coloured by chain, side view.
PDB entry 3r4t coloured by chain, top view.
Source organism: Mycobacterium marinum M
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reactions catalysed: 
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-109587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 49.76 KDa
Source organism: Mycobacterium marinum M
Expression system: Escherichia coli
UniProt:
  • Canonical: B2HN70 (Residues: 2-445; Coverage: 100%)
Gene names: MMAR_2118, gabT
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MPD 1 x MPD
Ligand CL 4 x CL
Ligand NA 1 x NA
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P6222
Unit cell:
a: 123.62Å b: 123.62Å c: 157.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.191
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

3 mentions without citation

Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP-GABA adduct.
Bruce et al. (2012)
2 more