› 3r4y

X-ray diffraction
2Å resolution

Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) from Saccharophagus degradans 2-40

Released:
DOI: 10.2210/pdb3r4y/pdb
PDB entry 3r4y coloured by chain, front view.
PDB entry 3r4y coloured by chain, side view.
PDB entry 3r4y coloured by chain, top view.
Source organism: Saccharophagus degradans 2-40
Primary publication:
Crystal structure of a key enzyme in the agarolytic pathway, α-neoagarobiose hydrolase from Saccharophagus degradans 2-40.
Ha SC, Lee S, Lee J, Kim HT, Ko HJ, Kim KH, Choi IG
Biochem Biophys Res Commun 412 238-44 (2011)
PMID: 21810409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 32, N terminal Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 42.44 KDa
Source organism: Saccharophagus degradans 2-40
Expression system: Escherichia coli
UniProt:
  • Canonical: Q21HB2 (Residues: 1-368; Coverage: 100%)
Gene name: Sde_2657
Sequence domains: Glycosyl hydrolases family 43
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 129.87Å b: 76.81Å c: 90.11Å
α: 90° β: 101.86° γ: 90°
R-values:
R R work R free
0.164 0.161 0.206
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Agar degradation by microorganisms and agar-degrading enzymes.
Chi et al. (2012)
6 more

1 mention without citation

A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66T Encoded in a Sizeable Polysaccharide Utilization Locus.
Schultz-Johansen et al. (2018)