› 3r5g

X-ray diffraction
1.5Å resolution

Crystal structure of the adenylyl cyclase CyaB from P. aeruginosa

Released:
DOI: 10.2210/pdb3r5g/pdb
PDB entry 3r5g coloured by chain, front view.
PDB entry 3r5g coloured by chain, side view.
PDB entry 3r5g coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa.
Topal H, Fulcher NB, Bitterman J, Salazar E, Buck J, Levin LR, Cann MJ, Wolfgang MC, Steegborn C
J Mol Biol 416 271-86 (2012)
PMID: 22226839

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate cyclase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.11 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZ23 (Residues: 220-416; Coverage: 43%)
Gene names: PA3217, cyaB
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P2
Unit cell:
a: 51.36Å b: 36.31Å c: 94.76Å
α: 90° β: 98.76° γ: 90°
R-values:
R R work R free
0.188 0.186 0.23
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biological roles of cAMP: variations on a theme in the different kingdoms of life.
Gancedo JM. (2013)
5 more

4 mentions without citation

Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.
Tal et al. (2021)
3 more