› 3r5h

X-ray diffraction
2.2Å resolution

Crystal Structure of ADP-AIR complex of purK: N5-carboxyaminoimidazole ribonucleotide synthetase

Released:
DOI: 10.2210/pdb3r5h/pdb
PDB entry 3r5h coloured by chain, front view.
PDB entry 3r5h coloured by chain, side view.
PDB entry 3r5h coloured by chain, top view.
Source organism: Bacillus anthracis str. A0248
Primary publication:
Elucidation of the bicarbonate binding site and insights into the carboxylation mechanism of (N(5))-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.
Tuntland ML, Santarsiero BD, Johnson ME, Fung LW
Acta Crystallogr D Biol Crystallogr 70 3057-65 (2014)
PMID: 25372694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole carboxylase, ATPase subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.21 KDa
Source organism: Bacillus anthracis str. A0248
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand AIR 2 x AIR
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 57.442Å b: 82.785Å c: 168.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.248
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Catalytic and structural properties of ATP-dependent caprolactamase from Pseudomonas jessenii.
Marjanovic et al. (2021)

1 mention without citation

Identification and characterization of a hitherto unknown nucleotide-binding domain and an intricate interdomain regulation in HflX-a ribosome binding GTPase.
Jain et al. (2013)