› 3r7t

X-ray diffraction
2.3Å resolution

Crystal Structure of Adenylosuccinate Synthetase from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3r7t/pdb
PDB entry 3r7t coloured by chain, front view.
PDB entry 3r7t coloured by chain, side view.
PDB entry 3r7t coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Kim Y, Gu M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.88 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9PMG4 (Residues: 1-416; Coverage: 100%)
Gene names: Cj1498c, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CIT 1 x CIT
Ligand PGE 2 x PGE
Ligand PEG 2 x PEG
Ligand GOL 1 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6422
Unit cell:
a: 127.232Å b: 127.232Å c: 122.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli BL21

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