› 3r84

X-ray diffraction
2.05Å resolution

Structure of the Mediator head subcomplex Med11/22

Released:
DOI: 10.2210/pdb3r84/pdb
PDB entry 3r84 coloured by chain, front view.
PDB entry 3r84 coloured by chain, side view.
PDB entry 3r84 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.
Seizl M, Larivière L, Pfaffeneder T, Wenzeck L, Cramer P
Nucleic Acids Res 39 6291-304 (2011)
PMID: 21498544

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
hetero 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-152367 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 11 Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 86 amino acids
Theoretical weight: 10.13 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99278 (Residues: 5-89; Coverage: 74%)
Gene names: MED11, YM9718.11C, YMR112C
Sequence domains: Mediator complex protein
Structure domains: Helix Hairpins
Mediator of RNA polymerase II transcription subunit 22 Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 92 amino acids
Theoretical weight: 10.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32570 (Residues: 2-89; Coverage: 73%)
Gene names: MED22, SRB6, YBR1721, YBR253W
Sequence domains: Surfeit locus protein 5 subunit 22 of Mediator complex
Structure domains: Mediator of RNA polymerase II transcription subunit 22

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 148.49Å b: 173.79Å c: 101.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

The Mediator complex: a central integrator of transcription.
Allen et al. (2015)
14 more

2 mentions without citation

Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.
Seizl et al. (2011)
1 more