› 3r88

X-ray diffraction
1.73Å resolution

Anthranilate phosphoribosyltransferase (trpD) from Mycobacterium tuberculosis (complex with inhibitor ACS145)

Released:
DOI: 10.2210/pdb3r88/pdb
PDB entry 3r88 coloured by chain, front view.
PDB entry 3r88 coloured by chain, side view.
PDB entry 3r88 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The substrate capture mechanism of Mycobacterium tuberculosis anthranilate phosphoribosyltransferase provides a mode for inhibition.
Castell A, Short FL, Evans GL, Cookson TV, Bulloch EM, Joseph DD, Lee CE, Parker EJ, Baker EN, Lott JS
Biochemistry 52 1776-87 (2013)
PMID: 23363292

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 38.72 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX5 (Residues: 2-370; Coverage: 100%)
Gene names: MTCY190.03c, Rv2192c, trpD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PRP 2 x PRP
Ligand MG 4 x MG
Ligand GOL 1 x GOL
Ligand 14F 1 x 14F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2
Unit cell:
a: 94.312Å b: 78.849Å c: 100.494Å
α: 90° β: 110.27° γ: 90°
R-values:
R R work R free
0.177 0.175 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
3 more

3 mentions without citation

The tryptophan biosynthetic pathway is essential for Mycobacterium tuberculosis to cause disease.
Lott JS. (2020)
2 more