› 3r9f

X-ray diffraction
1.2Å resolution

Crystal structure of Microcin C7 self immunity acetyltransferase MccE in complex with Coenzyme A and Glutamyl sulfamoyl adenosine (ESA)

Released:
DOI: 10.2210/pdb3r9f/pdb
PDB entry 3r9f coloured by chain, front view.
PDB entry 3r9f coloured by chain, side view.
PDB entry 3r9f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for microcin C7 inactivation by the MccE acetyltransferase.
Agarwal V, Metlitskaya A, Severinov K, Nair SK
J Biol Chem 286 21295-303 (2011)
PMID: 21507941

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47510 (Residues: 338-521; Coverage: 35%)
Gene name: mccE
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand COA 2 x COA
Ligand GSU 2 x GSU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 78.979Å b: 94.804Å c: 53.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.2
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Bacterial GCN5-Related N-Acetyltransferases: From Resistance to Regulation.
Favrot et al. (2016)
10 more

3 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
2 more